Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase
Last updated on: 11-02-2022 - 10:29
Contact: Olivier De Wever
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Advances in cell culture methods allowed 3D culture of gut epithelial cells derived from adult intestinal epithelial stem cells in mice and humans. We show that starting from crypts cultured in an extracellular matrix hydrogel together with essential growth factors, gut organoids can be grown from
Last updated on: 20-01-2022 - 16:14
Contact: Bert Devriendt
Organisation: Ghent University (UGent)
Status: Still in development, Published in peer reviewed journal
Advances in three-dimensional (3D) culture techniques have shown several advantages over 2D cultures, especially by more accurately mimicking the in vivo environment. This has led to improved reproducibility and reliability of experimental results, which are important criteria in disease modelling
Last updated on: 14-10-2021 - 16:59
Contact: Jolanda van Hengel
Organisation: Ghent University (UGent)
Status: Still in development, Published in peer reviewed journal
The response of sublingual epithelial cells to house dust mite allergen and potential tolerance-promoting adjuvants such as Toll-like receptor (TLR) ligands and calcitriol was investigated using primary sublingual epithelial cells isolated from dogs and cultured in vitro. After 24-h incubation with
Last updated on: 07-04-2021 - 14:52
Retinal gene delivery via intravitreal injection is hampered by various physiological barriers present in the eye of which the vitreoretinal (VR) interface represents the most serious hurdle. We present a retinal explant model especially designed to study the role of this interface as a barrier for
Last updated on: 24-03-2020 - 16:26
Contact: Katrien Remaut
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Mononuclear cells are flushed from the bone marrow using a 25G needle. After centrifugation, the cells are resuspended in 50mL pre-heated DMEM containing 20ng/ml M-CSF. The cells are cultured in 10cm petri dishes for 7 days. At days 2,4 and 6, the medium is renewed after washing away non-adherent
Last updated on: 10-03-2020 - 10:17
Contact: Cannot be disclosed
Organisation: Ghent University (UGent)
Partners: Ghent University hospital (UZ Gent)
Status: Published in peer reviewed journal
We have developed an innovative two-chamber skin explant model specifically for studies involving skin of marine fish. An apparatus was 3D-printed and consists of two plates, a lower and upper plate (with funnel). By mounting the explant between the plates, two chambers can be created with the
Last updated on: 04-02-2020 - 13:31
Contact: Maaike Vercauteren
Organisation: Ghent University (UGent), University of Liège (ULiège)
Status: Internally validated
We describe a fluorescent cell-based in vitro infection model that reproduces host-Batrachochytrium dendrobatidis (Bd) interactions. Using the epithelial cell line A6 from Xenopus laevis, we reproduced different stages of host cell infection and intracellular growth of Bd, resulting in host cell
Last updated on: 10-01-2020 - 15:28
Contact: Elin Verbrugghe
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The BALB/c-derived - 4T1 mammary tumor cell line and RAW264.7 macrophage cell line were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin in culture flasks. Harvesting of cultured
Last updated on: 26-11-2019 - 13:12
Contact: Kristel Demeyere
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Epigenetics have taken centre stage in the study of diseases such as cancer, diabetes, and neurodegeneration. A new field is emerging that targets epigenetic modifications for therapeutic intervention: pharmacoepigenetics. Particularly in oncology, inhibitors of epigenetic-modifying proteins, i.e.
Last updated on: 12-11-2019 - 11:34
Contact: Maarten Dhaenens
Organisation: Ghent University (UGent)
Status: Still in development, Internally validated, Published in peer reviewed journal
