In vitro differentiation of hematopoietic stem cells (HSC) or inducible pluripotent stem cells (IPS) to megakaryocytes and platelets using specific differentiation conditions (liquid and 3D media). CRISPR/cas mutagenesis of HSC or IPS to study the effect of gene depletion or specific mutants on

Last updated on: 26-05-2022 - 10:41

Contact: Kathleen Freson
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Internally validated, Published in peer reviewed journal
The VITOTOX test is a bacterial genotoxicity test. The test is based on bacteria that contain the lux operon of Vibrio fischeri under transcriptional control of the mutated recN promoter, which is controlled by the bacterial SOS-system (TA 104-recN2-4 strain or Genox strain). After incubation

Last updated on: 26-05-2022 - 10:40

Contact: Roel Anthonissen
Organisation: Sciensano
Status: History of use, Published in peer reviewed journal
This method uses human skin-derived precursors (hSKP) differentiated towards hepatic cells (hSKP-HPC) as a hepatic in vitro model. Exposure of these cells for 24 hours to sub-cytotoxic concentrations of acetaminophen, which is a reference hepatotoxicant, induced specific cellular responses in a

Last updated on: 26-05-2022 - 10:39

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
The purpose of the present development is to use avian MoDCs to implement a cellular platform to increase understanding of the immune responses induced by various antigens of interest (e.g. vaccine candidates) and evaluate their immunogenic potential. Considering the difficulty to work on dendritic

Last updated on: 17-05-2022 - 17:00

Contact: Fiona Ingrao
Organisation: Sciensano
Status: Still in development
The CALUX® system (Chemically Actived LUciferase eXpression) of Bio Detection Systems (BDS, Amsterdam, the Netherlands) uses U-2 OS cells (human osteoblast) that are stably transfected with human TRb or human PPARg2 (BDS, Amsterdam) and a luciferase reporter construct under the control of a receptor

Last updated on: 05-05-2022 - 08:59

Contact: Cannot be disclosed
Organisation: Sciensano
Partners: BDS
Status: Published in peer reviewed journal
Recent advances in the development of human-based in vitro models offer new tools for drug screening and mechanistic investigations of new therapeutic agents. However, there is a lack of evidence that disease models respond favourably to potential drug candidates. Atopic dermatitis (AD) is a very

Last updated on: 22-04-2022 - 09:17

Contact: Christel Boudry
Organisation: straticell
Status: Internally validated, Published in peer reviewed journal
OECD TG 249 was developed by Prof. Dr. Kristin Schirmer, from Eawag. The OECD 249 aims at assessing the acute toxicity of single or mixtures of chemicals using the permanent rainbow trout gill cell line, RTgill-W1. RTgill-W1 cells in a confluent monolayer in 24-well plates are exposed to the test

Last updated on: 30-03-2022 - 17:15

Contact: João Barbosa
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The ATP cell viability assay (CellTiter-Glo assay test kit) provides a quantitative measurement of the ATP content directly proportional to the number of cells present in culture. The homogeneous assay procedure involves adding one single reagent, CellTiter-Glo Reagent directly to cells cultured in

Last updated on: 24-03-2022 - 11:20

Contact: Cannot be disclosed
Organisation: Sciensano
Status: History of use, Published in peer reviewed journal
The tetanus neurotoxin (TeNT) is one of the most toxic proteins known to man, which prior to the use of the vaccine against the TeNT producing bacteria Clostridium tetani, resulted in a 20% mortality rate upon infection. The clinical detrimental effects of tetanus have decreased immensely since the

Last updated on: 22-03-2022 - 16:49

Contact: Celine Vanhee
Organisation: Sciensano
Status: Published in peer reviewed journal
Lung cancer thrives in a complex multicellular tumor microenvironment (TME) that impacts tumor growth, metastasis, response, and resistance to therapy. While orthotopic murine lung cancer models can partly recapitulate this complexity, they do not resonate with high-throughput immunotherapeutic drug

Last updated on: 16-03-2022 - 14:53

Contact: Cleo Goyvaerts
Organisation: Vrije Universiteit Brussel (VUB)
Partners: Institut Curie
Status: Published in peer reviewed journal