We propose to generate three-dimensional tumoroids from the primary melanoma cell lines, as well as 3D organoids from the iPSc-derived neural crest stem cells. We will use the AggreWell system (STEMCELL Technologies) to generate uniform, size-controlled three-dimensional spheroids. After 5 days in
Last updated on: 25-02-2020 - 23:58
Contact: Valerie Christiaens
Organisation: VIB - KU Leuven
Status: Published in peer reviewed journal
Brain from treated and not treated mice is collected after death. Tissue is fixed in 4% PFA for 4 days. After specific cutting (ex. L and R hemisphere) tissue is placed in a cassette. Cassette is placed in Tissue Processor (where water from the tissue is removed and replaced with paraffin). Brain is
Last updated on: 04-03-2020 - 14:20
Contact: Cannot be disclosed
Organisation: Katholieke Universiteit Leuven (KUL)
Status: History of use, Internally validated
We have developed and validated yeast models to study aspects related to protein folding diseases like Alzheimer's and Parkinson's disease. These models allow to gain further insight in the cellular processes involved in the etiology of these disorders and as such identify potential new bio-markers
Last updated on: 11-02-2020 - 11:17
Contact: Joris Winderickx
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Patient-derived explants of prostate cancer provide an ex vivo model that retains the architecture and microenvironment of the native tissue. It enables the evaluation of drug responses on individual patient’s tumors ex vivo without passaging in animals. It is compatible with all molecular analysis
Last updated on: 07-02-2020 - 15:55
Contact: Johan Swinnen
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: University of Adelaide
Status: History of use, Published in peer reviewed journal
Ex vivo lung perfusion (EVLP) is a form of isolated lung perfusion in normothermic conditions and can be achieved with a pump-driven perfusion machine that recirculates a preservation solution through the vasculature of the lung in addition to mechanical ventilation. EVLP is based on a physiological
Last updated on: 07-02-2020 - 15:50
Contact: Arne Neyrinck
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Characterization of Human Oligodendroglia derived-EV-rich in sHSPs (HOG-EV-sHSPs). Establish HSPB1/B8 stably expressing cell lines for the production of sHSPs rich-EV cell culture conditioned medium. Human Oligodendroglia cell lines (HOG) were stably transduced with pLenti-HSPBs gene constructs
Last updated on: 14-08-2020 - 15:22
Contact: Joy Irobi
Organisation: University of Hasselt (UHasselt)
Status: Still in development, Internally validated, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
The method enables to quantitatively assess the invasion of endothelial cells in extracellular-matrix mimicking hydrogels, such as collagen or polyethylene glycol, and to measure the forces exerted by the cells that enable them to invade. Endothelial cells are seeded on the side of a hydrogel and
Last updated on: 11-02-2020 - 08:55
Contact: Hans Van Oosterwyck
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We have developed an innovative two-chamber skin explant model specifically for studies involving skin of marine fish. An apparatus was 3D-printed and consists of two plates, a lower and upper plate (with funnel). By mounting the explant between the plates, two chambers can be created with the
Last updated on: 04-02-2020 - 13:31
Contact: Maaike Vercauteren
Organisation: Ghent University (UGent), University of Liège (ULiège)
Status: Internally validated
Peripheral blood mononuclear cells (PBMC) are isolated from the peripheral blood using Ficoll gradient centrifugation. The PBMC are then stained with fluorescently-labelled monoclonal antibodies directed against cell surface or intracellular proteins that can be used to identify specific immune cell
Last updated on: 03-03-2020 - 11:46
Contact: Cannot be disclosed
Organisation: University of Hasselt (UHasselt)
Status: Published in peer reviewed journal
The GIDM-colon is a validated in vitro model that allows the study of the availability of compounds and the metabolisation at the level of the colon. The human physiological conditions of the gastrointestinal tract (stomach, small intestine and colon) are mimicked. The continiuous flow eliminates
Last updated on: 03-02-2020 - 08:44
Contact: Annelies Breynaert
Organisation: University of Antwerp (UAntwerpen)
Status: History of use, Internally validated, Published in peer reviewed journal
