Rat liver epithelial cells (rLEC) can be isolated from 8-day old male Sprague-Dawley rats. Briefly, small fragments of neonatal rat livers are incubated for 15 minutes with 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (HEPES) buffered trypsin solution [0.25 % (v/v)] and washed twice with

Last updated on: 08-05-2019 - 14:33

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal

This method describes the steps to go from a liver to a decellularized matrix. It uses mild and strong detergents to destroy cells and keep the extracellular matrix intact. This matrix can then be used for a variety of purposes, including (but not limited to) repopulation, basis for coating and

Last updated on: 08-05-2019 - 14:33

Contact: Paul Claes
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal

This method describes the steps from a living rat to a single cell solution of primary hepatocytes. This requires surgery on the lab animal, a perfusion with buffer solution, a digestion with collagenase and a filtration step to obtain primary hepatocytes.

Last updated on: 08-05-2019 - 14:33

Contact: Paul Claes
Organisation: Vrije Universiteit Brussel

This method describes a well-known optimised human in vitro model of drug-induced cholestasis. Cryopreserved primary human hepatocytes are cultured between two layers of extracellular matrix scaffold, which will delay dedifferentiation and allows to restore cell-extracellular matrix interactions.

Last updated on: 02-04-2019 - 10:45

Contact: Eva Gijbels
Organisation: Vrije Universiteit Brussel
Status: Still in development

This method uses human skin-derived precursors (hSKP) differentiated towards hepatic cells (hSKP-HPC) as an hepatic in vitro model. Exposure of these cells for 24 hours to sub-cytotoxic concentrations of acetaminophen, which is a reference hepatotoxicant, induced specific cellular responses in a

Last updated on: 27-03-2019 - 16:43

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal

HeLa cells are the first continuous cancer cell line and were isolated from the aggressive glandular cervical cancer of a 31-year old woman. It was the first aneuploid line derived from human tissue maintained in continuous cell culture. Knowledge of almost every process that occurs in human

Last updated on: 22-03-2019 - 11:39

Organisation: Vrije Universiteit Brussel
Status: Still in development, History of use

Human skin-derived adult stem cells differentiated towards hepatic cells (hSKP-HPC) are used in this method (R. M. Rodrigues et al., Stem Cells Dev. 23, 44–55 (2014)). These cells are exposed to a cocktail of insulin and glucose at certain concentrations. After 24h of exposure, these cells

Last updated on: 22-03-2019 - 11:39

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel
Status: Still in development

Non-alcoholic fatty liver disease (NAFLD) ranges from simple steatosis to severe, life-threatening non-alcoholic steatohepatitis (NASH). Steatosis is mostly asymptomatic and does not cause health complications. However, in 5-10% of the cases it proceeds to NASH in which hepatic inflammation

Last updated on: 22-03-2019 - 11:37

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel
Status: Still in development