The genetic model organism Caenorhabditis elegans as a model for neurobiology research
Scope of the method
- Animal health
- Human health
- Basic Research
- Education and training
- In vivo
- Animal derived cells / tissues / organs
- brain plasticity
- C. elegans
- molecular biology
The nematode Caenorhabditis elegans provides a powerful model system to study fundamental working mechanisms of the nervous system in a living animal. It's main advantages are its compact nervous system that has been fully mapped, its short generation time and amenability for genetic research. As a model system, C. elegans allows to rapidly dissect the molecular and cellular basis of neural signaling, brain plasticity and the neural circuits underlying behavior. Many of the genes and molecular machinery that are used by the nervous system to steer animal physiology and behavior are well conserved between C. elegans and other animals, including humans. This way, it offers several opportunities to unravel the neuronal functions of conserved genes and catalyze functional studies of these genetic pathways in other model systems. In addition, its small size and transparency makes this model ideally suited for high-throughput functional studies and translational research including drug target screening.
C. elegans is a microscopic animal so most handling and observation of this animal is done using standard and confocal microscope setups. Breeding and maintenance of the animal is low in cost, and comparable to microbiology culturing.
- Published in peer reviewed journal
Pros, cons & Future potential
The main advantages of C. elegans as a model system for neurobiology research are its compact nervous system that has been fully mapped, its short generation time and amenability for genetic research. Thanks to these features, C. elegans allows to rapidly dissect the molecular and cellular basis of neural signaling, brain plasticity and the neural circuits underlying behavior. It's small size also makes this model amenable to high-throughput screening.
Due to its small size, dissections of tissues or other material are challenging. However, this is compensated by its transparency that allows to visualise most processes using fluorescent reporters in vivo.
References, associated documents and other information
Beets, I. et al. Natural Variation in a Dendritic Scaffold Protein Remodels Experience-Dependent Plasticity by Altering Neuropeptide Expression. Neuron 105, 106–121.e10 (2020).
Peymen, K. et al. Myoinhibitory peptide signaling modulates aversive gustatory learning in Caenorhabditis elegans. PLoS Genet. 15, e1007945 (2019).
Van Sinay, E. et al. Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans. Proc. Natl. Acad. Sci. U.S.A. 114, E4065–E4074 (2017).
Beets, I. et al. Vasopressin/oxytocin-related signaling regulates gustatory associative learning in C. elegans. Science 338, 543–545 (2012).
Contact personIsabel Beets