The method detects two facets of drug-induced cytotoxicity i.e. the intracellular accumulation of phospholipids and of neutral lipids, i.e. phospholipidosis and steatosis respectively. The assay makes use of a kit containing an aqueous, red-fluorescent formulation of labelled phospholipids (LipidTOX
Last updated on: 14-08-2025 - 13:13
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
This method describes a very reliable and robust in vitro model for the screening of the cholestatic liability of drugs and other chemical entities. The 3D spheroids generated from primary human hepatocytes can be cultivated up to 28 days, allowing long-term exposures which can depict otherwise
Last updated on: 14-08-2025 - 13:09
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
Aim: To model cartilage-related disorders in a human-specific context for the investigation of disease mechanisms and the screening of potential therapeutics.
Technique: Fibroblasts and/or peripheral blood mononuclear cells (PBMCs) from both healthy donors and patients are reprogrammed into
Last updated on: 07-08-2025 - 15:33
Contact: Pauline De Kinderen
Organisation: University of Antwerp (UAntwerpen)
Status: Internally validated
Thoracic aortic aneurysm (TAA) involves the progressive enlargement of the thoracic aorta, posing a significant risk for life-threatening aortic dissection and/or rupture. Currently, mouse models are frequently used to investigate and target the molecular defects underlying TAA, due to the
Last updated on: 06-08-2025 - 14:40
Contact: Ivanna Fedoryshchenko
Organisation: University of Antwerp (UAntwerpen)
Status: Still in development
The effect of fluidics that mimic the blood flow in the liver sinusoids, is evaluated during the hepatic differentiation of human skin-derived precursors (hSKP). In a standard bi-dimensional (2D) cell culture system, hSKP are differentiated to hSKP-HPC for 24 days in static conditions. In a
Last updated on: 23-07-2025 - 09:51
Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
For the generation of the human skin-derived precursors (hSKP) spheroids, ultra-low attachment 96-well plates, cell-repellent (Greiner) are used. hSKP cultured on tissue culture plates are detached with trypple (5 minutes) and collected in a tube. After centrifugation, hSKP are counted at the Burker
Last updated on: 23-07-2025 - 09:50
Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
Drug-induced phospholipidosis (DIPL) is a metabolic disorder characterized by an excessive intracellular accumulation of phospholipids caused by cationic drugs. Hepatic cells derived from human skin are evaluated as an in vitro model to investigate DIPL and its mechanisms. Human skin stem cells
Last updated on: 23-07-2025 - 09:49
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
This method/PhD project explores an innovative approach using synthetic communities (SynComs) to develop vaginal therapeutics. First, a top-down approach will be used to screen and select consortia of vaginal microbiota, emphasizing synergistic interactions and eliminating antagonistic effects. A
Last updated on: 16-07-2025 - 10:51
Contact: Maline Victor
Organisation: University of Antwerp (UAntwerpen)
Status: Still in development
Generation of cortical organoids from patient and control hIPSC.
Last updated on: 16-07-2025 - 10:01
Contact: Sarah Weckhuysen
Organisation: Vlaams Instituut voor Biotechnologie (VIB), University of Antwerp (UAntwerpen)
Status: Internally validated
We generate iPSC derived neurons and astrocytes using NgN2, ASCL1 and SOX9 overexpression respectively.
Last updated on: 16-07-2025 - 10:00
Contact: Sarah Weckhuysen
Organisation: Vlaams Instituut voor Biotechnologie (VIB), University of Antwerp (UAntwerpen)
Status: Published in peer reviewed journal
